Simplified Scatchard plot assay for progesterone receptor in breast cancer: comparison with single-point and multipoint assay.
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چکیده
منابع مشابه
Simplified Scatchard-plot assay for estrogen receptor in human breast tumor.
In this two-point Scatchard-plot assay, with which a test of competitive inhibition is combined, the sample is mechanically homogenized in a buffer containing dithiothreitol, ultracentrifuged to obtain a fat-free cytosol, the protein content of which is then adjusted, and free and bound labeled estradiol are separated with dextran-coated charcoal after overnight incubation. We tested the method...
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Background: Breast cancer is the most common cancer in women, containing approximately one third of all illnesses in women. Assessment of molecular markers is valuable in predicting the outcome of disease and decision making for optimal treatment. The purpose of this study was to determine the relationship between estrogen and progesterone receptors with Her-2, Ki67, P53, and clinicopathologica...
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Breast cancer is a complicated disease that it is accompanied by different symptoms. Diagnosis of this disease is performed by various techniques. Using Radiopharmaceuticals is a new method to diagnose the said tumors. [18F]-FPTT is one of these nuclear medicines for detection of breast cancer. It seems that the binding of the title radiopharmaceutical to the progesterone receptor is...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1978
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/24.7.1170